An experiment was conducted ontwelve adult white leg horn layers birds in their late phase of laying cycle from the poultry farm unit No. 3, department of Poultry Science, Bombay Veterinary College Goregaon, Mumbai 65.Blood sampling was done early in the morning at 8am by using 5ml syringe. Blood sample from the experimental birds was collected at weekly intervals. Biochemical parameters viz total protein, albumin, glucose, triglyceride, cholesterol, calcium, phosphorus were estimated by using pri-test diagnostic ready made kit supplied by [ROBONIC (INDIA) PVT .LTD. Plot No .A 374, TTC, MIDC industrial Area, Navi Mumbai- 400710, INDIA] .The parameters which were evaluated include total protein, albumin, serum glucose, cholesterol,triglyceride, calcium and phosphorus. The glucose content of the birds decreased with the age and were: 297 ± 4.45; 245 ± 11.42; 171.59±6.58 and 165.13 ± 8.33 mg/dl at 74th, 75th, 76th and 77th week of age respectively while the other parameters like cholesterol, total protein, albumin and triglyceride increased with age. The total protein levels were: 6.43 ± 0.13gm/dl; 7.26 ± 0.53gm/dl; 8.08 ± 0.51 g/dl and 8.28 ± 0.51 g/dl at 74th week, 75th week, 76th week and 77th week of age respectively.Non statistical difference was found in calcium but variation in level of calcium was seen with increase of age during egg laying season. Phosphorus showed non significant variation during lying season but was found statistically significant (p<0.05)

Keyword: Layers chicken; Total protein; Albumin; Glucose;Triglyceride; Total cholesterol; Ca & P;