Parasitic infections are one of the major constraints for the profitable dairy industry in tropical and subtropical countries including India. The prevalence and intensity of parasitic infestation are primarily influenced by the wide range of agroclimatic conditions, animal husbandry practices, and pasture management practices. The current study was conducted in Rajasthan and Uttar Pradesh state in 2022 with the objective to assess the knowledge regarding management of parasitic infestation in dairy animals. The primary data were collected personally from 160 dairy farmers (comprising 80 Small Dairy Farmers (SDF) and 80 Large Dairy Farmers (LDF). An ex post facto research design was used in the study and an interview schedule was used to collect the data. A standardised knowledge test was administered which had 30 items under 7 dimensions. Findings revealed that overall, 61.25% of Small Dairy Farmers (SDF) possessed a medium level of knowledge, while 22.50% possessed a high level of knowledge. The Mean Rank Score (MRS) was found to be 16.70 out of 30 for SDF and 18.77 for Large Dairy Farmers (LDF), with an overall knowledge gap of 44.33% for SDF and 37.41% for LDF. The largest knowledge gap was identified in the dimension of pasture and grazing management, with a gap of 59.25% for SDF and 53.50% for LDF. The lowest knowledge gap was identified in the dimension of ectoparasites infestation management, with a gap of 36.56% for SDF and 30.93% for LDF. The primary roots of the poor level of knowledge were ignorance and failure to recognize parasites as health issues in animals. The gap can be narrowed down by imparting proper education and creating awareness regarding the scientific management of animals.