Polyhouse vegetable cultivation has gained significant importance in modern agriculture, offering controlled environment conditions that enhance crop productivity and reduce the impact of external factors. However, the successful implementation of polyhouse farming requires adequate knowledge and understanding of the specific cultivation practices involved. Therefore, the purpose of this study is to evaluate the farmer knowledge about polyhouse vegetable cultivation practices in Southern Rajasthan. The study was carried out in two districts of Rajasthan namely Bhilwara and Chittorgarh. Data was collected through surveys and interviews conducted with farmers in the region. To measure the knowledge level of the respondents a knowledge test was developed. All the aspect about polyhouse and production technology were included in the knowledge test. Each aspect was further divided into several sub question to find out the existing level of knowledge of respondents about polyhouse vegetable cultivation. The findings provide valuable insights into the knowledge gaps and areas that require focused training and support to promote sustainable and efficient polyhouse vegetable cultivation. The study clearly shows that out of one hundred respondents, majority of respondents (63.00%) were having medium level of knowledge whereas, 16.00 per cent respondents were having high level of knowledge and remaining 21.00 per cent respondents possessed low level of knowledge about polyhouse vegetable cultivation practices. The study reveal that reveals that “knowledge about components of polyhouse” (drip irrigation, fogger system, fertigation system and isolation chamber) was on the first rank with overall MPS 100.00. whereas farmers having least knowledge about "nematode infestation in polyhouse and its control methods", ranked fifteenth with MPS 28.50. Nematode infestation was a big problem for polyhouse owners but they were unaware about it hence, they should be trained in identification of infestation and preventive control measures.