Transition period, a period, three weeks before and three weeks after calving of dairy animals plays an important role in the overall reproductive and productive performance of dairy animals. The present study was aimed in validating e-learning information module on transition period of dairy animals in enhancing the knowledge of dairy farmers. The study was conducted in the two randomly selected districts of Uttar Pradesh state. Total 160 farmers along with 40 experts (scientists of ICAR and SAUs) and field officers from line department (Veterinary Officers) were selected as respondents. Based on the knowledge and information need of dairy farmers, a e-learning information module covering various aspects of transition period was designed and developed. Validation of the module revealed that the experts were highly satisfied with the existing characteristics of the modules. The effectiveness of this module was assessed on the basis of knowledge gain. The overall mean knowledge gain with regard to transition period through the e-learning information module was found to be 18.08 percent which was highest in case of pre partum and dry period management practices. The perceived feedback was collected in terms of satisfaction the results indicates that majority of the respondents were highly satisfied with the characteristics like simple in understanding information, relevant information on transition period and effectiveness in enhancing knowledge respectively. The rate of adoption of practices is highly correlated with socio economic factors like education, income, herd size, milk production etc. of the farmers.